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Figure 4. Change-point plots for the data in

Figure 3 . Incremental changes in E R between remineralization intervals reveals that within 7days values increased predominantly in the sloped zone of the lesion (∼70–140 µm), while the 7–14 day period introduced mineral at the surface and final period between 14 and 28 days contributed only minimally to the total regain of stiffness across the lesion.

Figure 3

https://doi.org/10.1371/journal.pone.0038852.g004

Typical MicroXCT™ images are shown for the artificial caries lesions ( Fig. 5a ) and the PILP remineralized lesions after 7, 14 and 28 days ( Fig. 5b–d ). Fig. 5a shows the highly demineralized region of the artificial caries lesion followed by a prominent increase in mineral with depth until the normal mineral level was reached. At this magnification the widened dentin tubules can be seen near the base of the lesion. At the left side of Fig. 5a the normal dentin that was protected from the demineralization treatment is evident. These images also show that the exposed surface was prepared nearly perpendicular to the tubule direction. MicroXCT™ images from the remineralized specimens revealed marked x-ray attenuation in the 7 day specimens ( Fig. 5b ), with further increases in the 14 ( Fig. 5c ) and 28 ( Fig. 5d ) day specimens with little difference apparent between the 14 and 28 day specimens.

Figure 5. Typical 2d reconstructions showing x-ray attenuation with depth in the lesions at different remineralization times.

a) control lesion (0 time) showing protected portion at the left, the severely demineralized region which gradually blends with normal dentin. Widened dentin tubules were apparent at the depth of the lesion. b) 7 day remineralization treatment seems more complete in the deeper part of the lesion. c) 14 day remineralization shows some unevenness in the remineralization, but much of the lesion appeared fully mineralized. d) 28 day remineralization was similar to 14 day specimens. Note there is no apparent shrinkage in any of the specimens as the imaging was done in fully hydrated tissue (see supplemental Figure 1 ). Dotted line shows approximate lesion depth, magnification bar = 100 µm. A movie at 10X, demonstrating widened tubules at the lesion site is also provided as supplemental information.

https://doi.org/10.1371/journal.pone.0038852.g005

Figure 6 shows the changes in mineral content at each time period of the PILP remineralization process based on analysis of the MicroXCT™ tomograms. The averaged mineral profile from MicroXCT™ indicated restoration of mineral starting from the deepest part of the lesion and providing nearly complete remineralization after 14 days with only minor additional gains in the following 14 days. It is interesting to note that the apparent depth of the lesions based on mineral profiles was about 110–120 µm, compared to the 140 µm that were apparent from the mechanical properties measurements (see Fig. 2 ). The major difference seems to be in the mineral content associated with the flat portion of the artificial lesion that is only about 40 µm, compared to about 70 µm in the flat portion as measured by nanoindentation. The sloped region in both cases is approximately 70 µm.

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+ how tarot is “essentially the greatest comic evermade”

Words by Madeleine Morley

Published onSeptember 1st, 2016

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Tarot is often associatedwith divination and mysticism. But historically, picture card packs had a variety of uses and styles, including storytelling: the combination of these pursuits is what makes contemporary creatives keep coming back to tarot design centuries later.

In the 14thcentury, wealthy families in Italy commissioned expensive artist-made decks known as “carte da trionfi” (“cards of triumph”), where the imagery paralleled the mechanics of a game. Randomly drawn cards would make a new taleevery time the game was played, and decisions of players influenced the story. In this way, tarot was like an early, illustrated choose-your-own-adventure.

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